Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Abstract

Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the<br />cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of<br />apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS<br />and L929 cells were cultured and treated with different concentrations (0–10 mg/ml) of Cornus mass L. extract for 48<br />and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified<br />at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and<br />double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by<br />ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation<br />among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in<br />comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately<br />several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC50 value in AGS cells (evaluated<br />after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry<br />indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each<br />of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation<br />in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.