Genetic Polymorphism of Drug Metabolizing Enzymes (CYP2E1, GSTP1) and Susceptibility to Bladder Cancer in North India

Abstract

Glutathione-S-transferases (GSTs) are active in the detoxification of wide variety of endogenous or exogenous ‍carcinogens and genetic polymorphisms of CYP2E1 and GSTP1 genes have been studied extensively to evaluate the ‍relative risk of various cancers. In the present study, we examined associations with CYP2E1 and GSTP1 gene ‍polymorphisms in sporadic bladder cancers from North Indian patients. The subjects were 106 bladder cancer (Ca- ‍B) cases and 162 age-matched controls. The GSTP1 313 A/G polymorphism was determined by the PCR/RFLP ‍method using peripheral blood DNA. Binary Logistic Regression Model was used for assessing differences in genotype ‍prevalence and their associations between patient and the control group. We observed a non-significant association ‍in Pst1 polymorphism of the CYP2E1 gene; though the A/G genotype (OR = 2.69, 95% CI=1.57- 4.59, P= 0.000) and ‍G/G genotype (OR = 7.68, 95% CI=2.77- 21.26, P= 0.000) of the GSTP1 gene polymorphism alone or in combination ‍with tobacco users were highly significant (OR=24.06; 95% CI: 4.80- 120.42; P =0.000) when compared to the ‍controls. The results of our study demonstrated that the GSTP1 313 G/G polymorphism is a strong predisposing ‍risk factor for bladder cancer in the North Indian population.