Assessment Production of Natural Reactive Oxygen Species Affected on Dormancy Alleviation, Germination and Antioxidant System in Sunflower Seeds

Abstract

<strong>BACKGROUND:</strong> The active oxygen species, despite the damaging effects they have useful roles in the body are living things.<br /> <strong>OBJECTIVES:</strong> This research was done to determine whether Reactive Oxygen Species (ROS) could mediate Cyanide and Methylviologen signal in seed dormancy alleviation and sunflower seed germination, more widely, to assess their putative role in the control of seed germination and antioxidant system.<br /> <strong>METHODS:</strong> The experiment was laid out in completely randomized design with four replications. The treatment consisted non-dormant seed, dormant seeds, dormant seeds treated with Methylviologen (producing reactive oxygen species) and dormant seeds treated with hydrogen Cyanide (gas producing reactive oxygen species). For germination test 25 seed take in 9cm petridishes on filter paper. For biochemical assay seeds were imbibed for 24h and therefore Hydrogen peroxide (H₂O₂), Malondialdehyde (MDA) and four antioxidant enzymes such as Catalase (CAT), Proxidase (POX), Ascorbat proxidase (APX) and Superoxide dismutase (SOD) ware measured.<br /> <strong>RESULT:</strong> Maximum seed germination was recorded at non-dormant seeds and minimum of it was measured at dormant seeds. Lowest H₂O₂ and MDA production and lowest APX activity was recorded at dormant seeds. However, highest three detoxified enzymes activities such as CAT, POX and SOD was founded at dormant seeds. This enzyme activity was coinciding with lowest ROS production such as H₂O₂ and MDA production. ROS production as resources such as Cyanide and Methylviologen play a key role in the control of sunflower dormancy alleviation and seed germination.<br /> <strong>CONCLUSION:</strong> Main reason for seeds dormancy alleviation is production of ROS is acceptable level so germination of dormant seeds which was treated with Methylviologen and Cyanide was more than dormant control seeds and was similar to non-dormant seeds.