ENZYMOLOGICAL CHARACTERISTICS OF PLASMA MEMBRANE PHOSPHATIDATE PHOSPHOHYDROLASE (PAP2) FROM RAT LIVER

Abstract

Phosphatidate phosphohydrolase (PAP2b, fraction b) was purified from the plasma membrane ofrat liver cells. The Km for the surface concentration of phosphatidic acid was 0.43 mol%. The subunit of theenzyme had an M.W. of 33.8 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Thenative enzyme shows a molecular weight of 182 kDa in a gel filtration column packed with Sephacryl S300 inthe presence of Triton X-100. The pH optima obtained for PAP2b were 5.5 and 7 in imidazole and Tris- HClbuffers, respectively. The membrane homogenate enzyme (PAP2) consumed the lamellar (La) phase ofphosphatidate and was activated (approximately 3-fold) by Lubrol PX, CTAB and Tween 80 and inhibited byZn2+ and Mn2+. The inhibition was concentration dependent. These cations affected PAP2b activity through the phase transition of phosphatidate from lamellar (La) to inverted hexagonal (HII) form. Guanidinehydrochloride and urea increased PAP2 activity (2-fold) up to 20mM concentrations by stabilizing the Laphase. Optimum activity of purified PAP2b was obtained at 3% trehalose and 7% sucrose. The data suggested that the stability of the La form of phosphatidate by detergent micelles may take place through surface dilution processes.