THE EFFECTS OF UV-A, UV-B AND UV-C ON PROTEIN AND ASCORBATE CONTENT, LIPID PEROXIDATION AND BIOSYNTHESIS OF SCREENING COMPOUNDS IN BRASSICA NAPUS

Abstract

The role of the antioxidant defense system was studied in Brassica napus, and subjected todifferent supplementary bands of ultraviolet radiation (UV-A, UV-B and UV-C). This study showed thatUV-B and UV-C induced oxidative stress as evidenced by an increase in lipid peroxidation and totalascorbate content. Under UV-B and UV-C treatments, flavonoids and anthocyanins were increasedsignificantly compared with control and UV-A treated plants. These compounds act as internal filtersand also have an antioxidant activity against active oxygen species such as H2O2. Results of this researchshowed that the protein content increased under UV-B and UV-C. These increments are probably relatedto the synthesis of defense proteins such as HSP, the expression of genes that are encoding theantioxidant enzymes, or those which contribute in UV-absorbing compound synthesis pathways such asPAL, CHS and CHI.