Evaluation of the TPS gene as an internal control gene in Gossypium genotype for the quantitative detection of cotton transgenic product using Time-Real PCR technique

Abstract

Nowadays 6 million farmers are planting transgenic plants in 16 various countries around the world. Currently, more than 27% of the cultivated transgenic plants are in 9 developing countries. Having attention to increased production of transgenic plants in the world, monitoring of these transgenic products has important in order to production, exporting and importing. In the current situation, it is important to design and implement the qualification and quantification tests in our country for tracing the transgenic plants due to the production of some of these plants such as rice and cotton. There are various diagnostic methods for monitoring transgenes, but the Real-time PCR is one of the most precise approaches among them. On the other hand, given that the application of this method based on using an internal control gene, the utilized gene is required to have specific characteristics and specifications. In this study, we succeeded in introducing the internal control gene of Trehalose 6- phosphate-synthase (TPS) for evaluating the transgenic cotton plant in the world for the first time.