Impact of the Anthropogenisation on the Metal Bioaccumulation and Distribution in the Spiny-Cheek Crayfish (Orconectes limosus Raf.) from Lake Gop³o, Poland

Abstract

This study was undertaken to determine the concentrations of metals in the muscle and exoskeleton<br />of the crayfish Orconectes limosus in order to compare them with other species of crayfish and crustaceans.<br />In addition, we analyzed the correlation between the concentrations of the same element in the exoskeleton and<br />the meat and different elements accumulated in the same tissue. Crayfish individuals were acquired in spring<br />(May 2012) from Lake Gop³o. Individuals with damaged claws were not analyzed. Due to the relatively low<br />amounts of meat that was obtained from the abdomen of individual crayfish, material from individuals with a<br />similar body length was combined; thus, there were ten samples of meat and the exoskeleton obtained on the<br />whole. The muscle samples (about 12 g) were freeze dried in a Finn-Aqua Lyovac GT2 freeze dryer (Finland).<br />Three replicates of each freeze-dried sample were acid digested with 1 ml of concentrated HNO3 and 1 cm3<br />H2O2. Metal analyses were performed using an inductively coupled plasma mass spectrometry (ICP-MS)<br />with a Perkin-Elmer Optima 8300 spectrometer. The analyses revealed that the mean metals concentrations<br />(mg·kg-1) in the meat of the crayfish and in the exoskeleton were in the following order (Zn (115.571) > Mn<br />(18.825) > Cu (17.226) > Ni (15.472) > Pb (3.535) > Cr (0.769) > Co (0.551) > Cd (0.315) > Hg (0.138)) and<br />(Mn (111.640) > Zn (11.355) > Ni (8.165) > Pb (6.695) > Co (0.595) > Cu (0.575) > Cd (0.379) > Cr (0.195)<br />> Hg (0.0168)), respectively.