UV-Visible spectrophotometry of Repaglinide in bulk and in formulation by using methyl orange as reagents

Abstract

Two sensitive spectrophotometric methods are presented for the assay of Repaglinide in bulk<br />drug and in formulations using methyl orange, as reagents. Oxidant by reacting with a fixed<br />amount of either methyl orange and measuring the absorbance at 485.2 nm (method-I) and<br />measuring the absorbance at 618.9 nm (method-II). Developed method is based on the formation<br />of extractable colored complex of drug with coloring agent Methyl orange dye. A wavelength<br />maximum was found to be 618.9 nm. The concentration range of 15-50 μg ml-1 with linear<br />regression of 0.9995, while the percentage recovery, LOD and LOQ were 99.42-99.08 %, 2.17<br />μg ml-1 and 1.08 μg ml-1 respectively. The result of analysis have been validated statistically and<br />also by recovery studies. From the percentage recovery and specificity studies it was concluded<br />that there was no interference of common additives during the estimation. This proves the<br />suitability of this method for the routine quality control analysis of the Repaglinide in<br />formulation.