Spectrophotometric determination of Dalacin phosphate from formulations using reduce atomic absorption of calcium ion by phosphate

Abstract

A simple, sensitive and accurate method for spectrophotometric determination of Dalacinphosphate<br />has been established. The method is based on the reduction of atomic absorption<br />calcium ion by phosphate. Because, there is a phosphate mole in every Dalacin mole structure<br />and with measurement of phosphate, Dalacin is beyond measurement too. Therefore, the reaction<br />between acidic hydrolysis products of antibiotics with the mixture of calcium ion evaluated for<br />the spectrophotometric determination of mentioned drug in formulations. Experimental<br />parameters related to the performance of atiomic absorption spectrometry (AAS), such as<br />concentration of calcium ion, reaction time and temperature were investigated and optimized.<br />Under the optimized conditions, the limit of detection (3σB) and relative standard deviation<br />(RSD, n=4) were 1.75 μg mL-1 and 1.4 %, respectively. The results obtained agreed with those<br />obtained by the USP method.