Simultaneous spectrophotometric determination of ampicillin and penicillin in human plasma using multivariate calibration

Abstract

An analytical methodology based on spectrophotometric and partial least squares (PLS) algorithm for the<br />simultaneous determination of ampicillin and penicillin in human plasma was developed and validated. The<br />multivariate model was developed as a binary calibration model and it was built and validated with an<br />independent set of synthesis and real samples in presence of matrix. It is shown how a developed technique<br />for signal filtering, orthogonal signal correction (OSC), can be applied in multivariate calibration to enhance<br />predictive power. The experimental calibration matrix was constructed with 25 samples. The concentration<br />ranges considered were 1.0-40.0 and 0.5-20.0 μg mL-1 for ampicillin and penicillin, respectively. This<br />procedure allows the simultaneous determination of ampicillin and penicillin in synthetic and human plasma<br />good reliability of the determination was proved. The results obtained by the OSC-PLS and HPLC were<br />statistically compared. Very similar values were found by two methods. No time consuming pretreatment<br />was needed and this method also provides rapid, accurate and economical analysis of these drugs.