Simultaneous spectrophotometric determination of lidocaine and hydrocortisone acetate in pharmaceutical preparations by multivariate calibration methods

Abstract

The multivariate calibration method, partial least square regression (PLS) was applied for the<br />simultaneous spectrophotometric determination of Lidocaine (LID) and Hydrocortisone acetate<br />(HCA) in their mixtures. The parameters of chemometric technique were optimized and the<br />proposed method was validated with synthetic samples and applied to analyze these drugs in<br />pharmaceutical products with good accuracy and precision. The results were compared with the<br />HPLC method. The squares of correlation coefficients (R2) for predicted LID and HCA with the<br />proposed method in test samples were 0.9970 and 0.9964, respectively. The relative standard<br />deviations for commercial products were less than 1%.