The measurement of the quercetin of different parts of Tribulus terrestris by HPLC

Abstract

Background and aims: <em>Tribulus terrestris</em> fruit, leaf, and root have medical effects in the treatment of cancer, viral infections and prevention of cardiovascular diseases. The present study was aimed to evaluate the quercetin flavonoid levels from different parts of the <em>Tribulus terrestris</em> collected from different regions of Khuzestan in 2014. Methods: In this experimental study, four parts of the <em>Tribulus terrestris</em> including; fruits, leaves, stems and roots were collected from different regions of Khuzestan including Shushtar, Mollasani and Andimeshk. The analysis was carried out to compare the chemical profile of the different extracts of <em>Tribulus terrorists</em> using reverse phase HPLC with UV detector. The mobile phase that consisted of phosphoric acid buffer with pH=3 and acetonitrile was used for isocratic elution. The flow rate was adjusted to 1.0 ml/min. The detection wavelength was at 203 nm. All separations were performed at ambient temperature. Results: The results reported that the quercetin flavonoid level were highest in the Andimeshk leaves samples (69.57427 ppm). However, the Andimeshk fruits samples (4.141953 ppm) have the lowest levels of the quercetin flavonoid. Conclusion: Considering the cost effectiveness in extracting compounds from medicinal plants, it is recommended to identify the highest level of the quercetin flavonoid in each region and in each part of the plant.