Comparison of Extraction and Measurement of Quercetin from Stigma, Style, Sepals, Petals and Stamen of Crocus Sativus by HPLC in Combination with Heat and Ultrasonic

Abstract

Flavonoids, are a large class of polyphenols, with more than 4000 combinations. They have an antioxidant role in plant photosynthesis and in the human body they optimally act as an antioxidant, anti-inflammatory, anticancer and protector of the heart. Quercetin is in the flavonol group that is used to fight protection specifically against viruses and cancer cells. In this study, Comparison of extraction and measurement of quercetin in combination with heat and Ultrasonic from Stigma, style, sepals, petals and stamen, of <em>Crocus sativus</em> L. by HPLC method was carried out. Samples were collected in last October 2012 from experiment farms, in a research station in Hamand Abesard in the east of Tehran, Iran. Various methods of extraction with the heat and Ultrasonic were tested using different organs (viz. Stigma, style, sepals, petals and stamen). A total of eight samples were obtained for measurement of quercetin by high-performance liquid chromatography (HPLC). In stigma with heat were 0.0856%, and with Ultrasonic were 0.0356%, in style with heat Soxhlet were 0.001%, and with Ultrasonic were 0.009%, in sepals and petals with heat were 0.409%, and with Ultrasonic were 0.307%, and in stamen with heat were 0.009%, and with Ultrasonic were 0.005%.