Total Flavonoids Contents and Anti Bacterial Activity of the Extracts of two Labiateae Species: Nepeta menthoides and Thymus trautvetteri

Abstract

Total flavonoids contents and antibacterial activity of the extracts from the aerial parts of two Labiateae species, <em>Nepeta menthoides</em> Boiss. & Buhse and <em>Thymus trautvetteri</em> Klokov & Desj.-Shost. were determined. Quantitative determination of flavonoid contents was calculated in terms of quercetin equivalent in various extracts (50% methanol, chloroform and distilled water) by AlCl₃colorimetric method. It was found that total flavonoids contents of <em>N. menthoides</em> extracts in different solvents were (2.308%, 0.884% and 0.710%) mg/g and (2.076%, 1.468% and 1.412%) mg/g for <em>T. trautvetteri </em>respectively.Antibacterial activity of the extracts was also determined against 6 gr (+/-) bacteria by disc diffusion method. The results showed that 50% methanol extracts of two species displayed better inhibitor activity against the tested bacteria. The bacteria <em>Stapylococcus epidermidis,</em> <em>Staphylococcus saprophyticus</em> and <em>Staphylococcus aureus</em> were sensitive against 50% methanol extract of <em>N. menthoides</em> even more than the standard antibiotics (10μg). Therefore a good correlation between total flavonoids contents and antibacterial activity of the studied extracts is demonstrated.