Evaluation of Antioxidant, Antibacterial and Photo catalytic Effect of Silver Nanoparticles from Methanolic Extract of Coleus Vettiveroids – an Endemic Species

Abstract

Biosynthesis of metal nanoparticles using plant extract has received much attention due to its eco-friendly nature. The present study elucidates the green synthesize of Silver nanoparticles (AgNPs) from methanolic extract of Coleus Vettiveroids –an endemic species. The synthesis of AgNPs was confirmed by UV-visible spectrometry at 416 nm. Further, biosynthesized nanoparticles were characterized by FTIR for the confirmation of biomolecules acting as reducing agent. Average size and presence of elemental silver were characterized by scanning electron microscopy (SEM) and Transmission electron microscopy (TEM).Average size of nanoparticles was found to be 5 nm. The antioxidant ability of AgNPs was analyzed using DPPH. In vitro antibacterial effect of various concentrations of AgNPs was investigated against both Gram positive (S.Aureus) and Gram negative (E.Coli) bacterial strains. The result shows that biosynthesized AgNPs have significant antibacterial activity. Synthesized silver nanoparticles were also used effectively as photo catalyst in degradation of Organic Dyes and can be concluded that synthesized silver nanoparticles are also promising photo catalyst.