Influence of Explant Nodal Positions on the In Vitro Shoot Regeneration of Rose

Abstract

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca' and ‘El Torro'. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca' 3.75; ‘El Torro' 2.65) were obtained from the explants distal to the apex than those of the proximal position with 5.0 mg/l BA. But proliferating shoots derived from the fifth nodal explant with 1.0 mg/l attained highest shoot length (‘Bianca' 1.43 cm; ‘El Torro' 1.19 cm) and produced higher number of leaves (‘Bianca' 5.45; ‘El Torro' 6.30) and fresh weight (‘Bianca' 659.38 mg; ‘El Torro' 255.95 mg) per explant than the third and fourth nodal explants. The fifth nodal explant with 1.0 mg/l BA was found the best treatment for the shoot regeneration of rose cvs. ‘Bianca' and ‘El Torro'.