Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector

Abstract

T-2 toxin is the most poisonous trichothecene produced by <em>Fusarium</em> species especially <em>F.sporotrichioides</em>. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in <em>Fusarium</em> species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11<em>Fusarium</em>isolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns" purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.