STREPTOMYCES FRADIAE, A POTENT PRODUCER OF CHOLESTEROL OXIDASE

Abstract

Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and conditions for enzyme production. The optimum media was found (g/Lit): glucose, 12; starch, 9; yeast extract, 6 ;peptone, 4; (NH ) SO ,7.5; Tween 80,0.5 with cholesterol, 2 as an enzyme inducer. 3 4 ?C w as the optlmum temperature for the production of cholesterol oxidase