SEPARATION OF POLYCYCLIC AROMATIC HYDROCARBONS WITH MIXED MICELLAR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Separation of Polycyclic Aromatic Hydrocarbons was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, types and amounts of coorganic solvent in mobile phase on efficiency, asymmetry factor and selectivity factor showed that the appropriate condition for separation of these compounds was 3% isopropanol (V/V) as co-organic solvent in mobile phase at 40°C. Analysis of the tridimensional plots of variation of SDS si.d Brij-35 concentration on selectivity factor showed that the optimum conditions were 45-65 mM of SDS and 1-1.5 mM of Brij- 35 in micellar mobile phase