THE EFFECT OF IONIC STRENGTH ON THE BINDING OF SODIUM N-DODECYL SULPHATE TO ASPERGILLUS NIGER CATALASE

Abstract

The binding of sodium n-dodecyl sulphate (SDS) to Aspergillus niger catalase in various ionic strengths at pH 6.4 and a temperature of 27? and 37?C has been studied over a range of SDS concentrations using equilibrium dialysis and fluorescence spectroscopy techniques. The binding data were used and interpreted in terms of theoretical models (Scatchard Equation and Wyman Binding Potential). The cationic ionizable residues were estimated and have been compared with data from amino acid analysis