LPS Induces microRNAs -9, -192, and -205 Overexpression in Colorectal Cell Lines SW480, HCT116

Abstract

<strong>Background:</strong> Today, cancer is one of the serious health issues worldwide. In USA, colorectal cancer is the third cause of death. Reportedly, some risk factors, including family history of colorectal cancer, age, and infectious diseases can deteriorate the progression of colorectal cancer. One of the most common specifications in all gram-negative bacteria is bacterial cell wall, known lipopolysaccharide. Moreover, LPS probably can affect some microRNAs and cancer progression. We studied the effects of LPS on microRNA-9, -192, and -205 expressions in colorectal cell lines [SW480, HCT116], which are considered oncogene.<br /> <strong>Methods:</strong> SW480 and HCT116 cell lines were treated with LPS to analyze microRNA-9, -192, and -205 expressions by quantitative real-time PCR in 48 hours at 10 ug/L of LPS.<br /> <strong>Results:</strong> Quantitative real-time PCR illustrated that microRNA-9, -192, and -205 were upregulated after treating LPS. There was an increase in microRNA-9 level, six and eight times in SW480 and HCT116 cell lines, respectively. Furthermore, upregulation in the expression of microRNA-192, six times in HCT116 and four times in SW480 was observed. Moreover, there was an upregulation expression [almost four times in both cell lines] in microRNA-205. Our results show that treating LPS increases microRNA-9, -192 and -205, which may be related to cancer in colorectal cell lines [SW480 and HCT116].<br /> <strong>Conclusion:</strong> Therefore, disrupting the balance of bacterial flora can be influential in colorectal cancer progression and increase the chances of getting colorectal cancer that further investigation is required.