Comparing size particle, release study and cytotoxicity activity of PHMB encapsulated in different liposomal formulations: neutral and cationic liposomes

Abstract

Charges in liposome composition plays a critical role, so it has significant outcome on the manner of liposome <em>in vitro </em>and <em>in vivo</em>. Neutral and cationic liposomes (LPF_S and CLPF_S) were prepared and characteristics of LPF_S and CLPF_S were studied after PHMB encapsulation. According to results, CLPF_S exhibited higher cytotoxicity activity, while kept similar size distribution, encapsulation efficiency, lower prolonged retention release profile in comparison to LPF_S. CLFP_S represented more stable release due to its mutual repulsive force. MTT assay of the produced formulation was examined versus normal primary human skin fibroblast cells and free PHMB presented stronger cytotoxic activity when compared to both cationic and neutral liposomes. It was concluded that PHMB are more toxic to the body but encapsulation liposome specially in neutral liposome can reduce the toxic effect of them.