Design and bioinformatics analysis of novel biomimetic peptides as nanocarriers for gene transfer

Abstract

Objective(s):<em> <br/></em>The introduction of nucleic acids into cells for therapeutic objectives is significantly hindered by the size and charge of these molecules and therefore requires efficient vectors that assist cellular uptake. For several years great efforts have been devoted to the study of development of recombinant vectors based on biological domains with potential applications in gene therapy. Such vectors have been synthesized in genetically engineered approach, resulting in biomacromolecules with new properties that are not present in nature.<br/>Materials and Methods: <br/>In this study, we have designed new peptides using homology modeling with the purpose of overcoming the cell barriers for successful gene delivery through Bioinformatics tools. Three different carriers were designed and one of those with better score through Bioinformatics tools was cloned, expressed and its affinity for pDNA was monitored.<br/>Results: <br/>The resultszz demonstrated that the vector can effectively condense pDNAinto nanoparticles with the average sizes about 100 nm.<br/>Conclusion: <br/>We hope these peptides can overcome the biological barriers associated with gene transfer, and mediate efficient gene delivery.