The effect of diazinon exposure on hepatic tissue and enzymes in male frog Rana sridibunda

Abstract

The purpose of this study was to examine the acute and sub-acute effects of diazinon on<br />histopathological parameters of liver tissue and blood level of hepatic enzymes of male common<br />frog (Rana ridibunda). Frogs were assigned to 9 groups (n=8, meaning 8 frogs in each group):<br />control and treatment groups with 30, 60, 90 and 120 μg/L diazinon in water for one day or one<br />week. Hepatic enzymes containing alanine transaminase (ALT), aspartate aminotransferase<br />(AST), and alkaline phosphatase (ALP) were measured using biochemical kit. All ALP values<br />were significantly different between the control group and one-day and one-week diazinon<br />exposed groups. Hepatocyte changes were observed only for diazinon concentrations greater<br />than 60 μl after one week. The most important characteristics of the affected cells were<br />hyperplasia and chromatin formation. Results show that sub-lethal concentrations of diazinon<br />have an increasing effect on the blood level of hepatic enzymes, which probably indicated the<br />detoxification response of liver on the basis of diazinon sub-lethal effect model.