Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.)

Abstract

Septoria tritici blotch (STB), caused by the ascomycete fungus <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Mycosphaerella </span></span></em>graminicola <span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">(asexual stage: </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Septoria tritici</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">), is one of the most important foliar diseases </span></span>of wheat. In this research, quantitative expression analysis of five candidate genes for the induction of resistance to STB (<em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">PR-1</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Bsi</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Msr, Per</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, and </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Ppi</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">) was conducted in the wheat </span></span>cultivars ‘Seri 82' (susceptible) and ‘Frontana' (resistant). The study used a randomized complete block design with three replications and five time-points (0, 3, 6, 12, and 24hours after inoculation). Evaluation of the expression of five candidate genes by real-time PCR showed that expression of <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Per, pr-1, Bsi, Msr </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">genes was higher than 5, 3, 3 and 2 </span></span>fold in treatment <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">vs. </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">control samples in ‘Frontana' at 12 hours after inoculation </span></span>respectively. The results indicated the effectiveness of these genes in the conferral of resistance to STB in wheat.