Comparison of Solid Substrate and Submerged Fermentation for Chitosan Production by Aspergillus niger

Abstract

Production yield of solid-state (SSF) and submerged fermentation (SMF) on chitosan from Aspergillus niger was investigated. A. niger BBRC 20004 was grown on soybean residue and Sabouraud Dextrose Broth medium (2% glucose). Chitosan was extracted from the fungal mycelia using hot alkaline and acid treatment. Soybean residue at a moisture content of 37%, and 8.4 0.26% of nitrogen content produced the highest amount of chitosan, 17.053 0.95 g/kg dry substrate after 12 days. Also, chitosan was extracted from A. niger on Sabouraud Dextrose Broth medium in submerged fermentation (0.8455 g/l after 12 days of cultivation). The yield of chitosan isolated in SSF was about 15-20 times more than in SMF (Submerged Fermentation). Keyword: Chitosan; Soybean residue; Solid-State Fermentation (SSF); Submerged Fermentation (SMF); Sabouraud Dextrose Broth (SDB); Glucose; Aspergillus niger.