Phylogeny of Phytophthora and Phytopythium species associated with rice in Fars province (Iran)

Abstract

In order to investigate the Oomyceteous species of the rice paddy fields of Fars province (Iran), during 2013–15, infected roots and crowns together with soil around seedlings and irrigation water were sampled. Based on the morphological, morphometric and physiological studies along with phylogenetic analyses of internal transcribed spacer sequences based on neighbor joining method, two <em>Phytophthora </em>species from Clades 6 and 8, including <em>P. inundata</em>,and <em>P. pseudocryptogea</em> and three <em>Phytopythium </em>species from a distinct clade, including <em>Phpy. litorale</em>, <em>Phpy. ostracodes</em>, and <em>Phpy. sterile </em>were identified<em>.</em> <em>Phytopythium litorale</em>,and <em>Phpy. sterile </em>were distinguished through morphological studies along with phylogenetic analysis. <em>Phytopythium sterile</em> was also new to the Oomyceteous mycobiota of Iran. All species were reported for the first time in the world from rice rhizosphere.