Severity of COVID-19 infection correlation with IL-17 polymorphism

Abstract

Single nucleotide polymorphisms (SNPs) in many studied genes have been related to the severity of COVID-19. This study was carried out to investigate whether the polymorphisms of two SNPs (rs763780 and rs2275913) of the gene polymorphisms for IL-17 are connected to the COVID-19 severity. The effect of these polymorphisms on the levels of IL-17 and the relationship between the level of IL-17 and the severity of COVID-19 were also investigated. RT-PCR was performed to detect SARS-COV-2. Blood samples have been taken for analyses of IL-17 levels by ELISA test and Genomic DNA was extracted for genotyping. Genotyping was performed using Real Time –Polymerase Chain Reaction. The results indicated that the IL-17 level significantly increases in patients compared with control (healthy people) and there is a relationship between the severity of COVID-19 and IL-17 levels (p<0.01) between severity group. Mean ± SE of IL-17 was 67.99 ±2.05 ng/L for the control group, 147.60 ±3.34 for mild or moderate, (218.15 ±6.27) for the severe group and (283.97 ±5.59) for the critical group. And there is no-significant Relationship between SNP80 and IL-17 level (ng/L) with different types of severity and there is no-significant Relationship between SNP13 and IL-17 level (ng/L) with different types of severity (p ≤ 0.05) both of them. The severity of COVID-19 and the prevalence of the AA genotype were shown to be significantly correlated. Nevertheless, rs2275913 A-allele carriers in the patients were shown to be at risk (P = 0.021) comparison with control group, show increase risk in the severe group (p-value= 0.005, p>0.05) and critical group (p-value = 0.023, p>0.05) but no compact between the mild group, There is no relationship (p< 0.05) between the prevalence of the GA genotype and the severity of COVID-19.