Metabolite Profiling of Avocado Seed Extract by LC-QToF-MS/MS: A Natural Approach to Enhancing Bone Healing

Abstract

Avocado is a plant that easily grows in Southeast Asia, especially in Indonesia. Research on the biological foundations of medicinal plant treatment qualities has become popular in recent years. Avocado seeds usually become organic waste that is not utilized, but they can reduce inflammation and increase wound healing after tooth extraction. More polyphenols are found in avocado seeds than in flesh or skin. There is no study about metabolite profiling on avocado seed that lives in Indonesia, especially in Kediri City East Java. This study aims to prove the potential effect of avocado seeds for wound healing by increasing woven bone formation after tooth extraction. This study is experimental research in vivo and analyzes metabolite profiles of avocado seeds using LC-QToF-MS/MS. The research samples were 20 white male Rattus norvegicus and were divided into two groups: control (K) and treated (KE). Then, the lower left incicives of the samples were extracted for H&E staining to analyze the woven bone area at days 7 and 14. The results LC-QToF-MS/MS show the avocado seed extract found many major compounds including catechin, cyanidin, chlorogenic acid, and quinic acid. The results show that the woven bone area was significantly higher in the treatment group. The conclusion is that Avocado seeds have a potential compound that can enhance bone formation