Green Synthesis of Silver Nanoparticles from Glycine max Pods: Potential Anti-Cancer Activity Against Hepatocellular Carcinoma

Abstract

Introduction: Hepatocellular carcinoma, represents one of the greatest burdens in the oncology domain, with a significant negative global health and mortality rates. Glycine max, a plant used for centuries in various traditional medicine approaches, has shown good anti-cancer potential. The main objective of study was to analyse the anti-cancer activity of nanoparticle of Glycine max .Methods: Nanoparticles were synthesized with Glycine max pods extract and a silver nitrate aqueous solution. Moreover, these are characterized via SEM-EDX, UV, XRD, and DLS analysis. Cell viability and cytotoxicity of bioinspired AgNPs was accessed using MTT as well as the trypan blue assay.Results: Structural alterations of HepG2 cells were analysed by phase contrast microscope, showing significant results at a dose of 30 μg/ml. The upregulated expression of Annexin-V protein confirms that cell death occurred through apoptosis .Conclusion: Glycine max has demonstrated its capability to produce AgNPs with apoptotic activities in vitro, offering a promising and economical solution for potentially combating hepatocellular carcinoma.