Selective and stable adhesion of Yeast cells to Boronate-containing Polymer Grafted to glass Supports

Abstract

A series of sorbents containing various amounts of borate groups were prepared by chemical immobilization of<br />P-aminoethylphenylboric acid on a copolymer- treated organosilane spacer glass plates. It was found that<br />baker's yeast cells selectively adhered to these supports. Yeast cells stained with procion red HE-3B formed<br />denser and more stable layer on the copolymer-grafted supports compared onto supports modified with<br />phenylboric acid via a organosilane spacer. Adhesion of yeast cells to boronate-containing polymer fixed on<br />solid support in presence of different concentration of fructose, a sugar with high affinity to boronates, was<br />studied.