The Iportance of Agglutination tests versus ELISA in the diagnosis of brucellosis

Abstract

Background: Brucellosis is one of the endemic and a Zoonotic disease of the Iran. Science the rapid diagnosis and quick treatment are very important, selection of a sensitive and specific test solving this problem is of a great importance. The main aim of this study is comparison of routine serologic test with standard Elisa test. Materials and methods: In this survey, 176 suspected patients with conformed clinical signs, were selected and them routine diagnostic test like Wright (rapid), Standard tube agglutination (STA), Coombs Wright test and selective and quantitative Elisa test was performed for all sample referred to diagnostic laboratory. Findings: out of 176 sample, 92 (52.27%) were male and 84 (47.73%) female. On performing Wright (rapid), standard tube agglutination (STA), Coombs Wright and Elisa, 45 (25.57%), 49 (27.84%), 58 (32.95%) and 72 (40.91%) sample were positive respectively. The range of age distribution was 15-65 years. The geographical distribution of population is 72.4% rural and 27.6% of urban category. The sensitivity of above mentioned test as compare to Elisa was 62.5, 68.0 and 80.0 percent respectively with 100% of specificity for all. Conclusions: The Elisa posses the highest diagnostic value as compare to other routinely performed tests. After Elisa other test namely Coombs Wright, standard tube agglutination and Wright (rapid) respectively. Have the highest diagnostic value with consideration to above findings, it is obvious that due to ability to find quantitative value, determination of antibody classis and stage of disease, Elisa is the best method of choice in diagnosis of brucellosis.