نمایش مختصر رکورد

dc.contributor.authorDucha, Nuren_US
dc.contributor.authorHariani, Dyahen_US
dc.contributor.authorBudijastuti, Widowatien_US
dc.contributor.authorSusilawati, Trinilen_US
dc.contributor.authorAulanni’am, Aulanni’amen_US
dc.contributor.authorWahyuningsih, Srien_US
dc.date.accessioned1402-04-06T13:23:27Zfa_IR
dc.date.accessioned2023-06-27T13:23:28Z
dc.date.available1402-04-06T13:23:27Zfa_IR
dc.date.available2023-06-27T13:23:28Z
dc.date.issued2023-04-01en_US
dc.date.issued1402-01-12fa_IR
dc.date.submitted2022-01-07en_US
dc.date.submitted1400-10-17fa_IR
dc.identifier.citationDucha, Nur, Hariani, Dyah, Budijastuti, Widowati, Susilawati, Trinil, Aulanni’am, Aulanni’am, Wahyuningsih, Sri. (2023). Effects of Adding α-tocopherol to Brahman Bull Chilled Semen on Sperm Quality, Lipid Peroxidation, Membrane Integrity, and DNA Integrity. Iranian Journal of Veterinary Science and Technology, 15(1), 31-40. doi: 10.22067/ijvst.2023.74571.1108en_US
dc.identifier.urihttps://dx.doi.org/10.22067/ijvst.2023.74571.1108
dc.identifier.urihttps://ijvst.um.ac.ir/article_43757.html
dc.identifier.urihttps://iranjournals.nlai.ir/handle/123456789/994897
dc.description.abstractDuring storage at low temperatures, the spermatozoa quality changes due to cold shock and free radicals. Diluent supplementation with antioxidants is an effort to maintain the quality of spermatozoa during storage. This study aimed to evaluate the α-tocopherol effect in CEP extender on sperm quality, membrane integrity, and lipid peroxidation during storage at 4°C-5°C. This was a laboratory experiment that compared the use of 2 mM of α-tocopherol in CEP with no addition of α-tocopherol (as control) in five bulls. Semen was collected from Brahman bulls, diluted in CEP with and without α-tocopherol, and stored at 4°C-5°C. Sperm motility and viability were investigated by a light microscope at a ×400 magnification using Eosin-Nigrosin staining. Moreover, membrane integrity was evaluated by lipid peroxidation using the MDA assay and hypoosmotic swelling test. The sperm motility, viability, and membrane integrity were higher in CEP with α-tocopherol. Lipid peroxidation was significantly different between the treatment and control groups. The α-tocopherol supplementation in the diluent CEP could maintain the spermatozoa quality during storage at 4°C-5°C.en_US
dc.format.extent439
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoen_US
dc.relation.ispartofIranian Journal of Veterinary Science and Technologyen_US
dc.relation.isversionofhttps://dx.doi.org/10.22067/ijvst.2023.74571.1108
dc.subjectα-tocopherolen_US
dc.subjectBrahman bullen_US
dc.subjectCauda epididymal plasmaen_US
dc.subjectFrozenen_US
dc.subjectSpermatozoaen_US
dc.subjectTheriogenologyen_US
dc.titleEffects of Adding α-tocopherol to Brahman Bull Chilled Semen on Sperm Quality, Lipid Peroxidation, Membrane Integrity, and DNA Integrityen_US
dc.typeTexten_US
dc.typeResearch Articleen_US
dc.contributor.departmentBiology Department, Faculty of Mathematics and Science, State University of Surabaya, Surabaya, East Java, Indonesia.en_US
dc.contributor.departmentBiology Department, Faculty of Mathematics and Science, State University of Surabaya, Surabaya, East Java, Indonesia.en_US
dc.contributor.departmentBiology Department, Faculty of Mathematics and Science, State University of Surabaya, Surabaya, East Java, Indonesia.en_US
dc.contributor.departmentReproduction Laboratory, Faculty of Animal Husbandry, Brawijaya University, Malang, East Java, Indonesiaen_US
dc.contributor.departmentBiochemistry Laboratory, Faculty of Veterinary Medicine, Brawijaya University, Malang, East Java, Indonesia.en_US
dc.contributor.departmentReproduction Laboratory, Faculty of Animal Husbandry, Brawijaya University, Malang, East Java, Indonesia.en_US
dc.citation.volume15
dc.citation.issue1
dc.citation.spage31
dc.citation.epage40
nlai.contributor.orcid0000-0003-0836-2930


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