Chemical Analysis of Volatile Constituents of Pulicaria Mauritanica Isolated by Hydrodistillation and Headspace Solid-phase Micro-extraction Techniques. Antimicrobial Activity of its Essential Oil

Abstract

The objective of this study is to report the comparative chemical analysis of essential oil (EO) obtained by hydrodistillation (HD) and volatile fraction (VF) detected by headspace solid phase micro-extraction (HS–SPME) isolated from Pulicaria mauritanica using Gas Chromatography–Retention Indices (GC-RI) and GC–Mass Spectrometry (GC-MS) and to evaluate in vitro the antimicrobial activity of P. mauritanica EO against three bacterial strains referenced by the ATCC (American Type Culture Collection): Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) and a one yeast Candida albicans using the paper disc diffusion and minimum inhibitory concentration (MIC) assays. The chromatographic profile indicates that quantitative and semi qualitative differences between the chemical compositions of both analysed samples were observed. The antimicrobial activity tested by two methods indicates that this EO was more effective against three strains tested excepted P. aeruginosa which was most resistant. Indeed, the results of the report MBC (Minimum Bactericidal Concentration)/MIC show that the EO has a bactericidal effect on S. aureus and a bacteriostatic and fungistatic effect on E. coli and C. albicans, respectively. P. mauritanica EO seems be a promising source of natural products with potential antimicrobial activity.