Design and Characterization of New Nano System for Delivery of Erythropoietin

Abstract

Erythropoietin (EPO) can increase cell maintenance during different damages of central nervous system as a strong cell protector. However, studies displayed that brain-blood barrier prevents the entrance of large proteins similar to EPO (30.4kD) into the brain. The goal of this study was to find an alternative method for delivering of EPO to the brain to skip the blood brain barrier using design of new nanodrug delivery system. Then, anew cationic Gemini surfactant has been used in this study for preparation of EPO-loaded Gemini micelles. The physic chemical properties of nano micelles was investigated using Dynamic light scattering (DLS) and ζ-potential measurement, Morphology studies and in vitro drug release. Preparation of EPO loaded Gemini micelles results indicate that there are appropriate interactions between EPO drug and Gemini micelles and that of the EPO loaded in to the Gemini nanoparticles. DLS results display that size of the Gemini-EPO nanoparticle is smaller than 150 nm. Morphology studies results have further shown that, micelles (EPO-loaded) having smooth regular surface and the sizes of TEM micrograph were reported between 100-150 nm. Also, in vitro drug release results indicate that nanomicells (EPO-loaded) has been degraded in simulated early-time periods and released the drug into a simulated solution results also indicate decreasing stability structure of EPO-Gemini against temperature. <br /> In conclusion, the obtained results proposed that Gemini as a Nano carrier can bind to EPO with smooth surface micelles and size of 114 nm and can be considered as a candidate for drug delivery (EPO).