Polymorphism of SCD1 and DGAT1 Gene in Isfahan Holstein Cows

Abstract

In this study we estimated the allele and genotype frequencies of SCD1 and DGAT1 gene polymorphism. The analysis was conducted on 408 Holstein cows from five dairy herds in Isfahan province. Genotypes were identified using PCR-RFLP technique. Two genetic variants (A and V) of the SCD1 gene were observed in this experiment. The frequency of A-allele ranged from 0.49 to 0.66, while frequency of V-allele 0.34 to 0.51. Regards with DGAT1 gene, also two genetic variants (A and K) were determined that frequency of A-allele ranged from 0.54 to 0.68 and K-allele 0.32 to 0.46. It was found that these two genes were polymorphic in Isfahan Holstein cows, which suggested that could be associated with composition and production traits