Cloning, expression and purification of Clostridium botulinum neurotoxin type E binding domain

Abstract

Botulinum neurotoxins constitute a family of bacterial toxins for botulism syndrome in human. The toxins<br />bind with high affinity to nerve cells where they cause a complete inhibition and release of neurotransmitters<br />and thereby produce flaccid paralysis. In this study the binding domain of type E neurotoxin was isolated by<br />PCR and expressed in a proper expression vector. The results of this investigation can be used as a tool<br />to study the mechanism of binding of holotoxins. This study is also implicated in antibody production against<br />botulism syndrome.