Effect of Host Src-kinase Inhibition on Replication of Coxsackievirus B3 in vitro

Abstract

There are evidence showing a relationship between host Src kinase activation and viral (CVB3) replication, which are based on the observation that inhibition in the enzyme activity could result in inhibition of viral<br />replication. The present study assessed the effect of Src kinase inactivation on viral replication at different<br />stages of infection. It was observed that the Src kinase activity is necessary for the initiation of viral replication. In this study HeLa cell lines were treated with 5 and 10 μM herbimycin A (Src kinase inhibitor) with a time schedule of -90´, -60´, -30´, 0´, +15´, +30´, +45´, ...+210 minutes. All cultures were infected with CVB3 at zero-minute (+ve sign indicates that herbimycin A was added after infection with CVB3). The reaction was terminated after 24 h, cells were then detached from petri plates with trypsin/EDTA. Viral replication was<br />monitored using a set of specific primers and the plaque formation unit (PFU) count. In cells pretreated<br />with herbimycin A before infection viral replication was inhibited. However addition of herbimycin A after infection did not affect viral replication.