نمایش مختصر رکورد

dc.contributor.authorRashidi, Seyed Khalilen_US
dc.contributor.authorMousavi Gargari, Seyed Latifen_US
dc.contributor.authorEbrahimizadeh, Waleaden_US
dc.date.accessioned1399-07-08T20:19:47Zfa_IR
dc.date.accessioned2020-09-29T20:19:48Z
dc.date.available1399-07-08T20:19:47Zfa_IR
dc.date.available2020-09-29T20:19:48Z
dc.date.issued2017-04-01en_US
dc.date.issued1396-01-12fa_IR
dc.date.submitted2016-02-06en_US
dc.date.submitted1394-11-17fa_IR
dc.identifier.citationRashidi, Seyed Khalil, Mousavi Gargari, Seyed Latif, Ebrahimizadeh, Walead. (2017). Targeting Colorectal Cancer Cell Lines Using Nanobodies; AgSK1as a Potential Target. Iranian Journal of Biotechnology, 15(2), 78-86. doi: DOI:10.15171/ijb.1472en_US
dc.identifier.issn1728-3043
dc.identifier.issn2322-2921
dc.identifier.urihttps://dx.doi.org/DOI:10.15171/ijb.1472
dc.identifier.urihttp://www.ijbiotech.com/article_47020.html
dc.identifier.urihttps://iranjournals.nlai.ir/handle/123456789/85967
dc.description.abstractBackground: Colorectal cancer is the third most common type of aggressive cancers. Chemotherapy, surgery,<br />and radiotherapy are the common therapeutic options for treating this cancer. Due to the adverse side-eff ects of these methods, immunotherapy is considered as an appropriate alternative therapeutic option. Treatment through the application of monoclonal antibodies is considered as a novel alternative therapeutic method for cancers. The variable fragments of the antibodies' heavy chain or VHHs have a wide application in molecular biology and biotechnology. VHHs are compatible with the phage display technology which allows rapid and high throughput screening for antibodies isolation.<br /><strong>Objectives:</strong> We aimed to use naive VHH phage library to isolate a specifi c nanobody against colorectal tumor<br />associated antigen; the AgSK1.<br /><strong>Materials and Methods:</strong> In this research, naive VHH phage library was panned against two colorectal cell lines;<br />Ls174T and HT29 expressing diff erent levels of AgSK1 tumor associated marker. The high affi nity binders were selected and subcloned for higher expression levels of the VHH. The affi nity and specifi city of the isolated VHH were tested using ELISA. The reactivity of the VHH toward cancer cells was analyzed by competitive ELISA applying sera isolated from colorectal cancer patients.<br /><strong>Results:</strong> Results show that the isolated VHH recognizes and binds to the colorectal cancer cells with a high<br />affi nity. Moreover, the isolated nanobody is able to compete with the antibodies in the patient sera for the binding to the cancer cells.<br /><strong>Conclusions:</strong> Results suggest that this nanobody has a specifi c reaction toward colorectal cells and can be used for further investigation on the tumor associated antigens or production of mimotopes useful for immunotherapy.en_US
dc.format.extent516
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoen_US
dc.publisherNational Institute of Genetic Engineering and Biotechnologyen_US
dc.relation.ispartofIranian Journal of Biotechnologyen_US
dc.relation.isversionofhttps://dx.doi.org/DOI:10.15171/ijb.1472
dc.subjectAgSk1en_US
dc.subjectCell-panningen_US
dc.subjectcolorectal canceren_US
dc.subjectVHH nanobodyen_US
dc.subjectMolecular Biotechnologyen_US
dc.titleTargeting Colorectal Cancer Cell Lines Using Nanobodies; AgSK1as a Potential Targeten_US
dc.typeTexten_US
dc.typeResearch Paperen_US
dc.contributor.departmentDepartment of Biology, Faculty of Basic Science, Shahed University, Tehran, Iranen_US
dc.contributor.departmentDepartment of Biology, Faculty of Basic Science, Shahed University, Tehran, Iranen_US
dc.contributor.departmentDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.en_US
dc.citation.volume15
dc.citation.issue2
dc.citation.spage78
dc.citation.epage86


فایل‌های این مورد

Thumbnail

این مورد در مجموعه‌های زیر وجود دارد:

نمایش مختصر رکورد