In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B

Abstract

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective protein<br />expression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.<br />This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genes<br />that was used for cloning in E.coli. The etx and cpb genes were retrieved from the GenBank and a fusion<br />gene was designed to produce a chimeric fusion protein. Secondary and tertiary structures and specificities<br />of fusion protein were determined by online software. Results showed that the designed fusion gene construction is suitable for chimeric fusion protein expression.