Increasing in the Extraction Yield of Environmentally Friendly Antifouling Agent from Pseudomonas Aeruginosa MUT3 by Response Surface Methodology (RSM)

Abstract

<em>In the present study, the solvent-solvent extraction of phenazine 1-carboxylic acid (PCA) as an environmentally friendly antifouling agent from pseudomonas aeruginosa MUT3 culture was investigated. Accordingly, after screening the extraction ability of various solvents, </em><em>the combined effects of operating parameters such as solvent type (ethyl acetate, dichloromethane, and</em><em> n-hexane), solvent percent and mixing time on the PCA extraction process were analyzed using response surface methodology (RSM). As a consequence, ethyl acetate showed higher extraction yield (68%) and the optimum condition for PCA extraction were identified as 150% of solvent and 120 min mixing time. Meanwhile, the extraction yields for dichloromethane and n-hexane <br /> were measured by HPLC assay around 48.75 and 25.2%, respectively. The accuracy </em><em>of the obtained model was proved by 99.90% R² and 99.84% Adj R². In addition, the disk diffusion test </em><em>showed 9.2, 8 and 7.3 mm inhibition zone for ethyl acetate, dichloromethane and n-hexane, respectively. Consequently, the present study provided a great insight into the solvent-solvent extraction of antibiotics from the fermentation broth.</em>