Synthesis and Characterization of Nanoparticles Propolis Using Beeswax

Abstract

<em>In order to protection, convenient release, and increase of antibacterial of capsules to the treatment of diseases</em><em>, propolis nanoparticles encapsulate. Beeswax is used for covering because of its special physical</em><em> and chemical properties, ineffective and inactivity and ease of mixing</em><em>with materials without any adverse reaction. In this study, nanotechnology and renewable natural compounds of beeswax were used in the process</em><em> of encapsulating for protection against adverse environmental conditions. At first, propolis nanoparticles were mixed with</em><em>chloroform then ammonia buffer and Tween -80 was added to it while stirring with speed rpm 300. The mixture was shocked to form the capsule. After filtration and</em><em>washing produced capsules were dried </em><em>for 48 hours at room temperature. Assessment of formation and performance of the capsules was done</em><em> by changing parameters such as</em><em> pH, time and temperature, the loading of nanoparticles by spectrophotometry</em><em> method and increasing the antimicrobial properties using microbial</em><em> culture. Also, FT-IR analysis was done to prove the physical transplant of wax</em><em> and propolis. According to TEM images, the size of produced capsules was estimated</em><em> in the range of 200 to 500 nm with 95% distribution percentages. Based on Taguchi testing, the optimum time, temperature and pH for the release of encapsulated nanoparticles were 10 minutes, 43ºC and 10, respectively.</em>