Selective Extraction and Determination of Di(2-ethylhexyl) Phthalate in Aqueous Solution by HPLC Coupled with Molecularly Imprinted Solid-phase Extraction

Abstract

Surface Molecularly Imprinted Polymer (SMIP) for selective adsorption of di(2-ethylhexyl) phthalate(DEHP) was prepared on the surface of silica gel which was modified by aminopropyltriethoxysilane and acryloyl chloride in a two-step method. The prepared SMIP was used to prepare Molecularly Imprinted Solid-Phase Extraction (MISPE) column for selective extraction<em> of DEHP from aqueous solution. The results suggested that MISPE column had high affinity</em><em> and excellent selectivity for DEHP. And adsorption property of MISPE column was significantly superior to the commercial C18 column. The MISPE column was reusable. An analytical method MISPE-HPLC was developed for extraction and determination of the DEHP residues </em><em>in environmental water samples. The results showed that the linear relationship between the peak area and </em><em>the concentration of DEHP were as follow: y=2354.6x+1663.5 with a good correlation coefficient (R²=0.9997) and the detection and quantification limits reached 0.012 mg/L and 0.039 mg/L, respectively. The spiked recoveries of DEHP in river water and lake water sample were in the range </em><em>from 93.3% to 102.3 % at the spiked level of 50~1000 μg/L. The Relative Standard Deviations (RSD)</em><em> were less than 5.2%.</em>