Lipoxygenase-1 Leaching from Soybean Flour Employing Stirred Tank Contactor

Abstract

<em>The present work examines leaching of </em><em>lipoxygenase</em><em>-1 from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered.The acetic acid pH: 5.2 increased the leaching of lipoxygenase- 1.The sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. The effect of agitator speed on the geometrically- scaled- up reactor has shown that similar amount of enzyme is leached at lower speed. The result is in para with the conventional system. The importance of operational period from 5 to 50 min on the enzyme leaching was evaluated and higher enzyme leaching obtained within 10 min. of operation. The Sigmaplot-6 for statistical verification and developing a correlation for the enzyme leaching was used.</em>