Media Selection for Poly(hydroxybutyrate) Production from Methanol by Methylobacterium Extorquens DSMZ 1340

Abstract

<em>Plackett-Burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of Methylobacterium extorquens DSMZ 1340. Among the studied variables, nitrogen and phosphorus sources, MgSO₄and most of the trace elements were found to be significant variables for PHB production from methanol. At best condition (based on PHB concentration), dry cell weight, PHB content and PHB concentration were 3.81 g/L, 21.23 %, and 0.809 g/L, respectively. It was also </em><em>found that</em><em> most of the trace elements and phosphorus sources were influential parameters on the growth of microorganism but the kind of nitrogen source was not. The experimental results showed that deficiencies of nitrogen sources (NH₄Cl and NH₄NO₃), phosphorus sources (K₂HPO₄ and Na₂HPO₄) and MgSO₄ in medium, increased PHB accumulation.</em>