Quantification of Aryltetralin Lignans in Linum album Organs and In Vitro Cultures

Abstract

A procedure was developed for rapid <em>in vitro</em> germination of <em>Linum album</em> seeds by scarification and exposing to gibberellic acid (GA₃) and kinetin (Kn). Concentrations of podophyllotoxin (PTOX) and related aryltetralin lignans α-peltatin, β-peltatin, 5'-demethoxy-6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin (MPTOX) in <em>L. album</em> fresh plant organs were determined by HPLC. A degree of variation was observed in lignan content in different plant parts and growth stages. It was found that PTOX is predominant in productive organs and leaves. The highest amount of PTOX (0.651 mg/100g dry wt.) and MPTOX (0.670 mg/100g dry wt.) and the total quantified lignans were found in the capsules. <em>In vitro </em>cultures were studied for lignans followed by high productive cell line<em> </em>selection. Calli cultures were more productive for MPTOX than PTOX, while suspension cells accumulate comparable amounts of PTOX and MPTOX. The highest amount of PTOX (0.301% mg/g dry wt.) was found in suspension originated immobilized cultures.