The Evaluation of Topical Administration of Lint bells Oil in Different Doses on Circular Excision Wound Healing in Experimental Models

Abstract

Objective- This study aimed to investigate the effect of topical administration of lint bells oil (LBOC) on wound contraction as well as healing process. Design- Experimental study. Animals- in order to follow-up current study, 60 Male Swiss albino mice were used. Procedures- Animals were randomly divided into 5 groups (NO=12) including; vehicle-recived negative control, pure oil-received and test group. The animals in treatment group subdivided into two groups as 2% cream-treated (LBOC 2%) and 4% cream-treated (LBOC 4%).Two circular full thickness skin defects were made, in both sides of the backbone, 1 cm away from the midline and 2 cm away from each other with a 5-mm-biopsy punch. Using graph paper, the Percentage of wound contraction measured at 3rd, 6th, 9th and 12th days after surgery. Tissue samples were obtained at the 3rd, 6th and12th days post-wounding, from all groups and stained with Masson's trichrome and studied under light microscope. Results-In LBOC treated groups, the healing process shortened significantly, which was revealed with rapid reduction of wound area. Histological observations revealed that, LBOC in form of 2% formulation remarkably reduced inflammatory cells infiltration, enhanced collagen deposition and facilitated the epithelialization versus other groups. Conclusion and Clinical Relevance-In conclusion, our data showed that lint bells oil, specially in 2% doses, promotes wound contraction ratio and facilitated the healing processes by inhibiting the inflammatory stage and stimulated the prolifrative phase by enhancing the fibroblasts distribution and/or proliferation.