The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.)

Abstract

Dog rose (<em>Rosa</em> <em>canina </em>L.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as <em>Rosa</em> <em>hybrid</em> and <em>Rosa</em> <em>floribunda</em>. <em>In</em> <em>vitro</em> propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and disease-free plants. Micropropagation of <em>Rosa</em> <em>canina </em>L<em>.</em> was revised, using its nodal segments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl^⁻¹), GA₃(0 and 0.5 mgl^⁻¹) and NAA (0 and 0.5 mgl^⁻¹) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferation stage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl^⁻¹) and ½ VS medium in rooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl^⁻¹BAP. Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl^⁻¹of NAA or IBA. The present study presents an <em>in</em> <em>vitro</em> protocol for <em>R. canina</em>.