نمایش مختصر رکورد

dc.contributor.authorNajafi Zarrini, Hamiden_US
dc.contributor.author. Fusetti, Fen_US
dc.contributor.authorElzenga, J.T.M.en_US
dc.contributor.authorLanfermeijer, F.C.en_US
dc.date.accessioned1399-07-08T19:18:42Zfa_IR
dc.date.accessioned2020-09-29T19:18:42Z
dc.date.available1399-07-08T19:18:42Zfa_IR
dc.date.available2020-09-29T19:18:42Z
dc.date.issued2012-12-01en_US
dc.date.issued1391-09-11fa_IR
dc.date.submitted2011-03-01en_US
dc.date.submitted1389-12-10fa_IR
dc.identifier.citationNajafi Zarrini, Hamid, . Fusetti, F, Elzenga, J.T.M., Lanfermeijer, F.C.. (2012). Arabidopsis leaf plasma membrane proteome using a gel free method: Focus on receptor–like kinases. Journal of Plant Molecular Breeding, 1(1), 25-33. doi: 10.22058/jpmb.2013.1703en_US
dc.identifier.issn2322-3332
dc.identifier.issn2322-5092
dc.identifier.urihttps://dx.doi.org/10.22058/jpmb.2013.1703
dc.identifier.urihttp://www.jpmb-gabit.ir/article_1703.html
dc.identifier.urihttps://iranjournals.nlai.ir/handle/123456789/63850
dc.description.abstractThe hydrophobic proteins of plant plasma membrane still remain largely unknown.  For example in the <em>Arabidopsis </em>genome, receptor-like kinases (RLKs) are plasma membrane proteins, functioning as the primary receptors in the signaling of stress conditions, hormones and the presence of pathogens form a diverse family of over 610 genes. A limited number of these proteins have appeard in protein profiles. The detection of these proteins and thus the determination of their dynamics and tissue specificity, is technically challenging due to their low abundance and association to a lipid membrane. To identify new putative membrane proteins especially receptor systems, we used a gel free proteomic strategy based on mass spectrometry analyses of a plasma membrane fraction enriched in hydrophobic proteins. We produced from Arabidopsis leaf a highly purified plasma membrane fraction with the aqueous two-phase partitioning technique. By separating the proteins in the plasma membrane fraction with ion exchange and reverse phase chromatography and analyzing the resulting fractions on a MALDI-TOF mass spectrometer, over 900 proteins were detected. The plasma membrane proteome generated by this approach contains numerous plasma membrane integral proteins, one-third displaying at least four trans-membrane segments. An in silico analysis shows a correlation between the putative functions of the identified proteins and the expected roles for plasma membrane in transport, signaling, cellular traffic and metabolism. Of these proteins, 304 were annotated as membrane proteins, 69 were RLKs, distributed among the different receptor families in proportions reflecting the distribution in the genome. Of the RLKs that were identified, most are reported for the first time at the protein level and will constitute interesting targets for further functional studies.en_US
dc.format.extent519
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoen_US
dc.publisherGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT)en_US
dc.relation.ispartofJournal of Plant Molecular Breedingen_US
dc.relation.isversionofhttps://dx.doi.org/10.22058/jpmb.2013.1703
dc.subjectArabidopsisen_US
dc.subjectPlasma Membraneen_US
dc.subjectProteinen_US
dc.subjectReceptor-Like Kinaseen_US
dc.subjectMolecular markers & plant breedingen_US
dc.titleArabidopsis leaf plasma membrane proteome using a gel free method: Focus on receptor–like kinasesen_US
dc.typeTexten_US
dc.typeResearch Paperen_US
dc.contributor.departmentSari Agricultural Sciences and Natural Resources Universityen_US
dc.contributor.departmentUniversity of Groningenen_US
dc.contributor.departmentUniversity of Groningenen_US
dc.contributor.departmentSeed and Production Physiology Research, Syngenta Seedsen_US
dc.citation.volume1
dc.citation.issue1
dc.citation.spage25
dc.citation.epage33


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