Isolation and identification of antibacterial compound from common name Holothuria leucospilota in the Persian Gulf

Abstract

Echinodermata are one of the most important branches of invertebrate in marine ecosystems, which today are also known as a rich source of natural products with biological properties. Most of these properties belong to compounds with a terpenoid structure. According to the biological properties of sea cucumbers in the Persian Gulf, this scientific study investigates the antibacterial properties of lanosterol compounds extracted from Holothuria leucospilota of Hengam Island in Persian Gulf. The cucumber powder was extracted using acetone, then the extract purified by silica gel column chromatography with n-hexane and ethyl acetate combination to isolate the terpenoid compounds. Isolated compound were run through TLC and sprayed with vanillin-sulphuric acid reagent for detection of terpenoid compound and profile of isolated compound obtained by gas chromatography (GC). The lanosterol triterpenoid compound in column fraction was identified by GC. Antimicrobial properties were evaluated by dilution test on bacterial strain Escherichia coli, Pseudomonas aeruginosa, Klebsiella nemonya, Proteus vulgaris, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and Nocardia brazilinus. This compound has antibacterial effects on gram-positive bacteria (Bacillus subtilis, Bacillus cereus and Staphylococcus aureus). Antibacterial effects of the lanosterol indicate that it can be very effective in antibiotics production.